intronbio 25237
e-Myco? plus 支原體 PCR 檢測試劑盒
描述
干燥分裝 PreMix Kit 檢測包括致病性支原體在內的 209 種支原體
-
? 廣泛物種檢測
- 檢測 209 種支原體，包括致病性支原體
- 99% 支原體檢測 -
? 高特異性和靈敏度
- 應用熱啟動 Taq DNA 聚合酶
- 使用 CLP 技術設計的引物 -
? 物種檢測
- 可對擴增的 PCR 產物進行直接分型 -
? 采用有效的驗證系統
- 內部控制：它嵌入產品中，以防止錯誤的 PCR 測試可能引起的誤判
- 樣品陽性控制系統：確認模板的狀態 -
? 8-MOP 系統
- 8-MOP 解決方案可防止 PCR 產物攜帶污染 -
? 干燥和等分預混液，多合一系統
- 所有成分都在一個管中與干燥的沉淀條件預先混合
- 添加模板和引物后可以實施 PCR
- 凝膠加載染料已經預先混合用于直接電泳 -
? 高穩定性和重現性
- 干燥和等分的 PreMix 由 ALHP 系統控制
- 真空包裝系統可以防止氧化和潮濕
e-Myco? plus Mycoplasma PCR Detection Kit is Dried & aliquoted PreMix Kit to detect 209 kinds of mycoplasma including pathogenic mycoplasma. Mycoplasma is common and serious contaminants of cell cultures. It has been shown that 30% to 87% of cell cultures are infected with mycoplasma. Many mycoplasma contaminations, particularly in continuous cell lines, grow slowly and do not destroy host cells but are still able to affect various parameters, leading to unreliable or false results. These effects include changes in metabolism, growth, viability, DNA, RNA, and protein synthesis, and morphology. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotechnological products. e-Myco? plus Mycoplasma PCR Detection Kit is a set of primers designed to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Conventional techniques that are used to detect mycoplasma involve culturing samples on selective media, which needs at least 1 week to obtain results, whereas by performing PCR with this primer set, which is based on conserved 16S rRNA, detection results are obtained in a few hours. The adopted 8-methoxypsoralen (8-MOP) is used to extinguish the template activity of contaminated DNAs. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent interstrand crosslink after photo-activation by incident light at wavelength 320-400nm
應用
用于檢測細胞培養中*常見的支原體種類
套件內容
| 內容 | 8 項測試 | 48 次測試 |
|---|---|---|
| e-Myco? plus 支原體 PCR 檢測試劑盒？ | 8 管 | 48管 |
| DNase/RNase-free 蒸餾水 | 200 毫升 x 1 瓶 | 1 毫升 x 1 瓶 |
| 對照 DNA | 5 毫升 x 1 瓶 | 20 毫升 x 1 瓶 |
| 手動的 | 1個 | 1個 |
技術數據
通過三重檢查系統提高分析結果的可靠性
樣品控制：模板DNA條件和濃度參數
目標條帶：支原體感染參數
內控：檢測試劑盒PCR反應參數
e-Myco? plus支原體PCR檢測試劑盒的檢測限測試
為了確定基因組DNA的*小所需量，分離基因組DNA FR奧姆的M.發酵氨基酸球菌感染的K562細胞的純培養物。將分離的基因組 DNA 連續稀釋用于 PCR 檢測。結果表明，該試劑盒的檢測限為每次檢測 10 ~ 20 pg 基因組 DNA。
